Journal: Channels

Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice

doi: 10.1080/19336950.2025.2611704

Figure Lengend Snippet: Localization and expression of potassium channel-associated proteins in mouse atrial myocytes. A. Immunofluorescence images showing membrane localization and expression of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 in atrial cardiomyocytes from LFD and HFD groups. Scale bar, 100 μm. B. Representative Western blotting images and densitometric quantification of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 protein expression in atrial cardiomyocytes from LFD and HFD mice. LFD, low-fat diet; HFD, high-fat diet. Data are presented as mean ± SD ( n = 3). ns, not significant; * p < 0.05, ** p < 0.01 vs. LFD group.

Article Snippet: Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; InvitrogenTM, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA).

Techniques: Expressing, Immunofluorescence, Membrane, Western Blot

Journal: Frontiers in Oncology

Article Title: Heterotypic neutrophil-in-tumor structure: A novel pathological feature first discovered in the tissues of OPSCC

doi: 10.3389/fonc.2022.807597

Figure Lengend Snippet: General clinicopathological information of enrolled patients.

Article Snippet: All antibodies with working dilution ratio, company source and catalog number are listed as follows: anti-E-cadherin (1:1000 for western blotting (WB) and 1:200 for immune fluorescent staining (IF); BD Biosciences; BD610182), anti-CDKN2A (1:1000 for WB; Abcam; ab3642), anti-EGFP (1:1000 for WB; Invitrogen; MA1-952) anti-β-actin (1:5000 for WB; Sigma; A5441); anti-p16 (1:100 for IF; BOSTER; BM1592).

Techniques:

Journal: Frontiers in Oncology

Article Title: Heterotypic neutrophil-in-tumor structure: A novel pathological feature first discovered in the tissues of OPSCC

doi: 10.3389/fonc.2022.807597

Figure Lengend Snippet: Association between FNiT and clinicopathological characteristics.

Article Snippet: All antibodies with working dilution ratio, company source and catalog number are listed as follows: anti-E-cadherin (1:1000 for western blotting (WB) and 1:200 for immune fluorescent staining (IF); BD Biosciences; BD610182), anti-CDKN2A (1:1000 for WB; Abcam; ab3642), anti-EGFP (1:1000 for WB; Invitrogen; MA1-952) anti-β-actin (1:5000 for WB; Sigma; A5441); anti-p16 (1:100 for IF; BOSTER; BM1592).

Techniques:

Journal: Frontiers in Oncology

Article Title: Heterotypic neutrophil-in-tumor structure: A novel pathological feature first discovered in the tissues of OPSCC

doi: 10.3389/fonc.2022.807597

Figure Lengend Snippet: Univariate and multivariate analysis of the predictors for disease-specific survival in patients with OPSCC.

Article Snippet: All antibodies with working dilution ratio, company source and catalog number are listed as follows: anti-E-cadherin (1:1000 for western blotting (WB) and 1:200 for immune fluorescent staining (IF); BD Biosciences; BD610182), anti-CDKN2A (1:1000 for WB; Abcam; ab3642), anti-EGFP (1:1000 for WB; Invitrogen; MA1-952) anti-β-actin (1:5000 for WB; Sigma; A5441); anti-p16 (1:100 for IF; BOSTER; BM1592).

Techniques:

Journal: Frontiers in Oncology

Article Title: Heterotypic neutrophil-in-tumor structure: A novel pathological feature first discovered in the tissues of OPSCC

doi: 10.3389/fonc.2022.807597

Figure Lengend Snippet: In addition to hNiT, Disease stage, lymphovascular invasion and tumor grade as three independent predictors for DSS. (A) Disease-specific survival function in patients with different disease stages(p=0.008). (B) Disease-specific survival function in patients with different p16 statuses (p=0.004). (C) Disease-specific survival function in patients with different tumor grade (p=0.001).

Article Snippet: All antibodies with working dilution ratio, company source and catalog number are listed as follows: anti-E-cadherin (1:1000 for western blotting (WB) and 1:200 for immune fluorescent staining (IF); BD Biosciences; BD610182), anti-CDKN2A (1:1000 for WB; Abcam; ab3642), anti-EGFP (1:1000 for WB; Invitrogen; MA1-952) anti-β-actin (1:5000 for WB; Sigma; A5441); anti-p16 (1:100 for IF; BOSTER; BM1592).

Techniques:

Journal: Frontiers in Oncology

Article Title: Heterotypic neutrophil-in-tumor structure: A novel pathological feature first discovered in the tissues of OPSCC

doi: 10.3389/fonc.2022.807597

Figure Lengend Snippet: HNiT formation negatively correlates with p16 expression in OPSCC. (A) Representative images for p16 and E-cadherin staining in OPSCC tissues. In left image, OPSCC cells are significantly positive both in p16 and E-cadherin, while in right image, OPSCC cells are positive only in E-cadherin. Scale bars: 50μm. (B) Zoomed images of boxed regions by dashed line in (A) , right cartoons of each zoomed images outline the morphology of tumor cells or hNiT structure from the left image. (C) Expression intension of p16 in OPSCC patients of high FNiT(>=0.5%, n=91) and low FNiT(<0.5%, n=106). (D) Correlation analysis between p16 expression and FNiT (r=-0.5283, p<0.0001, n=197).

Article Snippet: All antibodies with working dilution ratio, company source and catalog number are listed as follows: anti-E-cadherin (1:1000 for western blotting (WB) and 1:200 for immune fluorescent staining (IF); BD Biosciences; BD610182), anti-CDKN2A (1:1000 for WB; Abcam; ab3642), anti-EGFP (1:1000 for WB; Invitrogen; MA1-952) anti-β-actin (1:5000 for WB; Sigma; A5441); anti-p16 (1:100 for IF; BOSTER; BM1592).

Techniques: Expressing, Staining